ISSN 1311-1477 (print); ISSN 131-3543 (online)
Bulgarian Journal of Veterinary Medicine
VOL. 12, No 4, DECEMBER 2009
CONTENTS
Published by the Faculty of Veterinary Medicine, Trakia
University,
6000 Stara Zagora, Bulgaria
Volume 12, Number 4, December 2009, Summaries
Mohammadpour, A. A., 2009. Investigations on the shape and size of molar and zygomatic salivary glands in shorthair domestic cats. Bulg. J. Vet. Med., 12, No 4, 221-225.
The investigation was carried out on five male adult domestic shorthair cats. The zygomatic gland in the cat was with an oval shape with indented borders. It was located on both sides of the head, between the dorsal-medial regions of the zygomatic arch, ventral region of the eyeball and laterally to the masseter muscle. The mean length of the gland was 8.10 ± 1.14 mm and 8.42 ± 0.99 mm for left and right sides respectively in rostrocaudal direction. The mean width of the gland was 19.40 ± 1.14 mm (left side) and 19.10 ± 0.89 mm (right side) in dorsoventral direction. The molar gland was elongated and located obliquely in the submucosal fascia of the inferior lip, near the oral commissure. The dorsal border of the molar gland was attached to masseter muscle and posterior facial nerve. The caudal end neighboured the anterior facial vein and the cranial end was close to the transverse jugular vein. The mean length of the molar gland was 20.40 ± 1.14 mm and 19.90 ± 1.14 mm and the mean width: 5.90 ± 0.74 mm and 5.80 ± 0.83 mm on the left and right side, respectively. There was no significant difference between the dimensions of molar and zygomatic salivary glands from left and right sides.
Key words: cat, molar salivary gland, morphology, zygomatic salivary gland
Ertas, H. B., E. Atil, G. Ozbey & H. B. Gulcu, 2009. Genotyping of Campylobacter jejuni isolates of poultry origin with different restriction enzymes. Bulg. J. Vet. Med., 12, No 4, 226-232.
In this study, 28 poultry-originating Campylobacter jejuni isolates were typed by fla typing. The restriction enzymes of AluI, DdeI, HinfI, EcoRI, and PstI were used alone or in combination. Six different profiles were obtained with AluI, DdeI and HinfI+DdeI combination. Seven, five and two different profiles were obtained with PstI, HinfI and EcoRI digestion, respectively. The PstI was found most discriminative for typing poultry-originated isolates.
Key words: Campylobacter jejuni, fla typing, poultry
Momtaz, H., 2009. Sequencing and phylogenetic analysis of infectious bovine rhinotracheitis virus gB and gD genes in Iran. Bulg. J. Vet. Med., 12, No 4, 233-239.
Bovine herpesvirus 1 (BHV-1) belongs to the Varicellovirus genus, Herpesviridae family and causes different respiratory, reproductive and nervous system disorders in cows, including infectious bovine rhinotracheitis (IBR). For determination of the genetic relationship of gB and gD – two of main BHV-1 glycoprotein genes in Iran with those in other countries, DNA fragments of 671 bp corresponding to gB and 1002 bp corresponding to gD from four IBR viral strains have been isolated from bovine respiratory disorders in Iran, then were amplified in PCR system, sequenced for determining nucleotide sequence and compared with identified nucleotide sequences of these genes in other countries. The results indicated 6.6% to 14.5% variability in gB gene and 1.1% to 13.3% variability in gD gene in four sequenced samples. A comparison made on gB and gD genes in Iran with other countries showed 6.4% to 59% variability in gB gene and 8.7% to 29.2% variability in gD gene.
Key words: bovine herpesvirus 1, gB and gD genes, Iran, phylogenetic relationship
Chaprazov, Ts. & I. Borissov, 2009. Time course of blood C-reactive protein and fibrinogen concentrations after experimental Pseudomonas infection in dogs. Bulg. J. Vet. Med., 12, No 4, 240-245.
An experimental infection was produced in six mongrel dogs from both genders, at the age of 6–7 years, weighing 16±2 kg, by intravenous injection of 24-hour broth culture of Pseudomonas aeruginosa containing 1.2×109 CFU/mL. Another six untreated dogs served as controls. Blood C-reactive protein (CRP) was assayed between baseline (hour 0) and post infection hour 24, whereas blood fibrinogen levels were determined between baseline and the 28th day after infection. It was found out that blood CRP concentrations were statistically significantly higher by the 4th hour of infection vs baseline (P<0.01), and at hours 6 and 24 vs controls as well (P<0.001). Fibrinogen alterations consisted in increased levels by hour 24 (4.25±0.23 g/L) vs baseline concentrations of 2.73±0.27 (P<0.01) of treated and 2.17±0.55 g/L in control dogs.
Key words: C-reactive protein, dogs, fibrinogen, Pseudomonas aeruginosa
Munir, M., M. Abubakar, M. T. Khan & S. H. Abro, 2009. Comparative efficacy of single radial haemolysis test and countercurrent immunoelectroosmophoresis with monoclonal antibodies-based competitive elisa for the serology of peste des petits ruminants in sheep and goats. Bulg. J. Vet. Med., 12, No 4, 246-253.
This project was conducted to investigate the comparative efficiency of competitive ELISA (cELISA), single radial haemolysis test (SRH) and countercurrent immunoelectroosmophoresis (CIEOP) for the diagnosis of Peste des Petits Ruminants (PPR) in sheep and goats. Serum samples from 198 sheep and 82 goats were collected from three different government livestock farms and all the samples were run simultaneously with three serological tests. The samples found positive for PPR antibodies through cELISA, SRH and CIEOP were 96 (34.2%), 55 (19.6%) and 67 (23.9%), respectively. Kappa statistics was used to evaluate the concordance between the laboratory-based test (cELISA) and field-based tests (SRH and CIEOP). Kappa statistics scores for cELISA vs SRH and CIEOP were 0.5851 (95% confidence interval 0.4848–0.6854) and 0.6668 (95% confidence interval 0.5733–0.7603), respectively, which indicate a “moderate” agreement between cELISA and SRH and “substantial” agreement between cELISA and CIEOP. SRH and CIEOP revealed a relative diagnostic sensitivity with cELISA of 54.1% and 64.5% and relative diagnostic specificity of 98.3% and 97.2%, respectively. The data suggested that for mass screening and control of PPR, these serological tests proved practical in the absence of cELISA since they have a high relative diagnostic specificity and a satisfactory relative diagnostic sensitivity.
Simeonov, K. B, G. V. Goujgoulova & N. D. Oreshkova, 2009. Serological survey on the prevalence of chicken anemia virus in backyard poultry flocks in Bulgaria. Bulg. J. Vet. Med., 12, No 4, 254-259.
A total of 529 serum samples from backyard chickens originating from 22 villages in Western Bulgaria were tested for presence of chicken anemia virus (CAV) antibodies by means of commercial enzyme-linked immunosorbent assay (ELISA) kit. Three hundred ninety one samples were submitted to the laboratory in a screening programme for surveillance of avian influenza (AI) and originated from birds with unknown production type, age and sex. Another set of 138 samples was collected for the purposes of the study as a representative part of the home-reared indigenous chickens in three villages (82 hens and 5 roosters above one-year old, and 51 chickens aged 4–18 weeks). CAV reagents were detected in all villages surveyed with seroprevalence rates ranging from 84.4% to 100%. Significant association between the seropositivity and age of birds tested was found, with more CAV reagents detected among adult hens and roosters than among chickens aged up to 18 weeks. The results from the study indicate that CAV is widespread amongst backyard poultry population in Bulgaria. However, additional investigations are necessary to evaluate the epidemiological impact of infection among home-reared chickens and productivity losses due to CAV-related immunosuppression.
Key words: antibodies, backyard chickens, chicken anemia virus, immunosuppression
Antonov, A. L., А. S. Atanassov & P. I. Georgiev, 2009. A modified technique for prolapsed fold excision in a bitch with vaginal hyperplasia. Bulg. J. Vet. Med., 12, No 4, 260-264.
A case of vaginal hyperplasia type III in a bitch during oestrus was reported in a 10-month female Turkish Kangal. Surgical excision of prolapsed fold was performed by means of a modified operative technique. No recurrence of the hyperplasia has observed during the subsequent oestrus.
Key words: bitch, operative excision, vaginal hyperplasia
Al-Swailem, A., M. A. Al-Dubaib, G. Al-Ghamdi, E. Al-Yamani, A. A. Al-Naeem, A. M. Al-Mejali, M. Shehata & O. M. Mahmoud, 2009. High sulphur content of water from deep bore wells as a possible cause of polioencephalitis in a camel. Bulg. J. Vet. Med., 12, No 4, 265-270.
A case of a neurological disease affecting adult camels (locally called Altair) was described. A female camel was presented with typical clinical signs: weakness of legs, continuous head shaking especially when the animal was stressed and wry neck. There were no respiratory, cardiovascular or digestive abnormalities. The she-camel ate and drank normally. Complete blood count results and liver and kidney functions were normal. No visible macroscopic lesions were seen in the carcass except for meningeal congestion. Microscopic examination of the brain showed malacic lesions and the meninges were congested and oedematous. The concentration of sulphur in the drinking water was 13.56 ±5.8 g/l which is considered very high for the nutrition of farm animals. The concentration of copper, molybdenum and selenium in water and feed were normal.
Key words: camels, deep bore wells, minerals, polioencephalomalacia, sulphur poisoning