Bulgarian Journal of Veterinary Medicine (BJVM)


ISSN 1311-1477

Bulgarian Journal of Veterinary Medicine
VOL.
8, No 3, SEPTEMBER, 2005

 

 CONTENTS

151

FULL TEXT

D. Vladova, M. Stefanov & Y. Toneva
Computed tomography study of thoracic
aorta in the cat

157

FULL TEXT

G. M. Sarov & T. I. Vlaykova
CHANGES IN BLOOD GLUCOSE, triglycerides
AND LIPID PEROXIDATION PRODUCTS IN RABBITS
AFTER HANGING FIXation

163

FULL TEXT

М. Р. POPOVA & C. S. POPOV
EFFECT OF CHEMICAL AGENTS ON SOME ENZYME ACTIVITIES AND ON THE STABILITY OF MEMBRANE STRUCTURES

173

FULL TEXT

I. Zarkov, I. Bochev, N. Oreshkova &  R. Manvell
ISOLATION OF AVIAN PARAMYXOVIRUS TYPE 1
(APMV-1) FROM free-living WILD mallards (ANAS PLATYRhYNCHOS). (Preliminary communication)

183

FULL TEXT

М. LYutskanov, v. urumova & v. petrov
Attempts for in vivo influence upon the resistance of Еscherichia Coli after treatment with flavophospholipol and economical traits of its administration under condition of artificial infection in chickens

193

FULL TEXT

R. Binev, R. Simeonov, R. Todorov & Y. Nikolov
Pathomorphological studies following experimental acute intoxication with the triazole fungicide triticonazole in pigs

199

FULL TEXT

K. Kalinova
Our Experience in the Surgical Treatment of the Multiorgan Echinococcosis

205

FULL TEXT

D. DINKOV, I. KANELOV, I. ZHELYAZKOVA & I. VASHIN
PERSISTENCE OF TETRACYCLINE AND OXYTETRACYCLINE IN BEE HONEY AFTER IMPROPER APPLICATION ON BEE FAMILIES
(
no summary is available)

Published by the Trakia University, Faculty of Veterinary Medicine,
6000 Stara Zagora, Bulgaria

 

 

Volume 8, Number 3, September 2005, Summaries

 

Vladova, D., M. Stefanov & Y. Toneva, 2005. Computed tomography study of thoracic aorta in the cat. Bulg. J. Vet. Med., 8, No 3, 151-156.

Computed tomography (СТ) with contrast enhancement allows a detailed anatomical description of feline thoracic aorta. For this purpose, 14 sexually mature clinically healthy domestic cats weighing 2.9 to 4.0 kg were investigated. The animals were anaesthetized and fixed in dorsal recumbency on a СТ table (Tomoscan, CX/Q, Siemens). Consequent transversal 5 mm cross-sections were made along the thoracic cavity, caudally to the thirds thoracic vertebra (Th3). The levels corresponding to the best representation of anatomical features of thoracic aorta were selected. The branches of the aortic arch (the brachycephalic trunk and  the left subclavian artery) were observed from the first studied Th3 segment to Th4. The aortic arch was detected at the Th5 level. The ascending aorta was visualized almost centrally to the Th6 segment, surrounded by the left atrium, the right atrium and the right ventricle. The descending aorta was observed on the left ventrally to the body of Th6. In caudal direction,  the descending aorta was positioned ventromedially to the vertebral column, that was the best visible at  Th10. This placement of the descending aorta was preserved up to aortic hiatus.

In conclusion, СТ is able to visualize in vivo thoracic aorta and allows a precise anatomical analysis for the needs of diagnostics.

Key words: cat, computed tomography (CT), thoracic aorta

 

Sarov, G. M. & T. I. Vlaykova, 2005. Changes in blood glucose, triglycerides and lipid peroxidation products in rabbits after hanging fixation.  Bulg. J. Vet. Med., 8, No 3, 157161.

Psycho-emotional stress is one of the risk factors for metabolic syndrome and related diseases. We decided to investigate the changes in glucose and lipids levels, as well as oxidative stress, generated after hanging fixation in rabbits. We used 12 male New Zealand White rabbits (2.5-3.5 kg body weight): 6 animals for hanging fixation and another 6 animals for fixation in box. Blood samples were taken before hanging and at min 15, 30, 90, 180 and serum levels of blood glucose and triglycerides (TG) were measured by commercially available kits. Lipid peroxidation products in plasma were evaluated before hanging and at minute 180 using the thiobarbituric acid method. Blood glucose level rose sharply after hanging (p=0.008) and remained at this level to the end of the experiment. TG also increased significantly after hanging (p=0.007), remained higher up to minute 90 and at minute 180 dropped down to the basal level (p=0.744). Significant increase in malonedialdehyde (MDA) after hanging fixation was also found (p=0.028). Hanging fixation appears to produce significant increase in MDA, blood glucose and triglyceride levels by a not well-established mechanism. Further research is needed to reveal these underlying mechanisms and the rationality of the use of the hanging fixation for investigation of stress-induced homeostatic alterations.

Key words: hanging fixation, malondialdehyde, serum glucose, serum triglycerides

 

Popova, M. P. & C. S. Popov, 2004. Effect of chemical agents on some enzyme activities and on the stability of membrane structures. Bulg. J. Vet. Med., 8, No 3, 163-171.

The effects of two chemical agents - sodium nitrite and phenobarbital (PB) applied separately or in combination, on the activity of enzymatic components of monooxigenase system of hepatocytes, the stability of liver lysosomes and peroxisomes as well as on the total activity of marker enzymes for these structures were investigated in male rats.

Following the combined administration of both agents, the effects of sodium nitrite predominated. Nitrite eliminated at a considerable extent the membrane-stabilizing effect of the barbiturate, decreased its activating influence on aniline hydroxylase and potentiated PB-induced activation on systems catalyzing lipid peroxidation.

Key words: NaNO2, phenobarbital, endoplasmic reticulum, enzyme activities, membrane stability

 

Zarkov, I., I. Bochev, N. Oreshkova &  R. Manvell, 2005. Isolation of avian paramyxovirus type 1 (APMV-1) from free-living mallards (Anas platyrhynchos) (Preliminary communication). Bulg. J. Vet. Med., 8, No 3, 173-181.

In our attempts of virus isolation we examined 236 samples (faecal, cloacal, intestinal content, visceral organs). The samples were obtained from 16 species of wild, free-living birds from 06.12.2003 to 25.03.2004. They were collected from 5 different areas, situated in Southeastern Bulgaria.

Viruses were isolated from the 3 consecutive passages, carried out on chicken embryos, in 5 samples (2.12% of the total number). All of them were from a single avian species (Anas platyrhynchos) and from one region.  

The isolates were defined by the haemagglutination test, followed by the haemagglutination inhibition test, as avian paramyxovirus type 1. In a consecutive study through RT-PCR carried out in the UK (Veterinary Laboratories Agency Weybridge, UK) they were related to the lentogenic representatives.

Key words: APMV-1, Anas platyrhynchos, ducks, mallards, virus isolation

 

Lyutskanov, М., V. Urumova & V. Petrov, 2005. Attempts for in vivo influence upon the resistance of Еscherichia coli after treatment with flavophospholipol and economical traits of its administration under condition of artificial infection in chickens. Bulg. J. Vet. Med., 8, No 3, 183-191.

In the conditions of experimental infection with a Е. сoli О2 strain, isolated from septic chickens and showing multiresistance to a variety of antimicrobial drugs, the effect of different doses of flavophospholipol (FPL) was surveyed. The possibilities for influencing the antibacterial resistance were followed up via monitoring the changes in growth inhibition zones in agar and the minimum inhibitory concentrations (MIC). The effect of the treatment on some economical traits - daily weight gain, feed conversion ratio etc. - was also determined.

It was found out that FPL had a relatively weak direct effect on parameters determining the resistance of the challenge strain. It was suggested that its inhibiting influence on the dissemination of the genetic determinants of resistance among microbial populations was rather more effective than that on the mechanisms of resistance, acting at the level of the microbial cell.

The features of FPL as growth promoter, acting effectively on weight gain and feed conversion ratio were confirmed.

Key words: feed conversion ratio, flavophospholipol, inhibition zones, minimum inhibitory concentrations (MIC), resistance, weight gain

 

Binev, R., R. Simeonov, R. Todorov & Y. Nikolov, 2005.  Pathomorphological studies following experimental acute intoxication with the triazole fungicide triticonazole in pigs. Bulg. J. Vet. Med., 8, No 3, 193-198.

The gross and microscopic pathomorphological changes in internal organs were investigated for evaluation of the toxic effect of the triazole fungicide triticonazole. One control and three experimental groups of pigs, treated orally via a gastric tube at doses of 0.2 g/kg (group I), 1 g/kg (group II) and 2 g/kg (group III) (0.1LD50, 0.5LD50 and LD50 respectively) were used. The pigs were euthanized 72 h after the treatment, a complete necropsy was performed and material for histological study was obtained. It was found out that the toxic effect of the triazole fungicide resulted in morphological changes in the liver (mononuclear proliferation, granular and initial toxic dystrophy), in lungs (peribronchial mononuclear proliferates), in lymph nodes (haemorrhages and lymphatic follicle activation), in pituitary gland (hyperaemia), in medulla oblongata (ballooned dystrophy of glial cells), in stomach (hyperaemia of gastric mucosa) and in kidneys (mononuclear proliferations among tubules and among nephrons).

Key words: macro- and microstructural changes, pesticide toxicity, swine, triazole fungicides, triticonazole

 

Kalinova, K., 2005. Our experience in the surgical treatment of the multiorgan echinococcosis. Bulg. J. Vet. Med., 8, No 3, 199-204.

The frequency of echinococcosis in Bulgaria has been increasing in the last decade which makes it a serious health problem. Fifty one patients with echinococcosis with more than one organ involved were treated at the department of General and Pediatric Surgery of University Hospital in Stara Zagora between 1989-2004. The lungs and liver were affected in 26 cases, both lungs in 13 cases, the liver and the spleen in 7 cases and the liver, spleen and lungs - in 5 cases.

The individual approach to the treatment of multiorgan echinococcosis was elective. Young patients with good general condition were treated in a single stage, while patients in poor general condition were treated in two stages.

Key words: multiorgan echinococcosis, single-stage procedure, thoracophrenotomy