Bulgarian Journal of Veterinary Medicine (BJVM)
ISSN 1311-1477
Bulgarian Journal of Veterinary Medicine
VOL. 6, No 4, DECEMBER, 2003
CONTENTS
Published by the Trakia University, Faculty of Veterinary Medicine,
6000 Stara Zagora, Bulgaria
Volume 6, Number 4, December 2003, Summaries
Atanasov, A. T., B. Tchorbanov, Zh. Tzokeva and V. Spasov, 2003. Isolation of a Galega officinalis L. fraction and a tentative evaluation of the chemical structure of biologically active substances, inhibiting platelet aggregation Bulg. J. Vet. Med., 6, No 4, 203-208.
A biologically active fraction (BAF) IV that inhibited platelet aggregation was isolated via elution from an aqueous extract of Galega officinalis L. and its chemical composition was tentatively determined. The aqueous extract was purified from impurities by column chromatography on Sephadex G-25 (BAF I), Sepharose 4B (BAF II), DEAE-Cellulose (BAF III) and Sephadex G-100 (БАФ IV). The maximum purified BAF IV fraction contained substanced with molecular mass of 100-140 kDa. The amino acid analysis and NIR spectrometry showed that the fraction contained 19-23% protein and about 74% polysaccharides. The consecutively isolated fractions inhibited platelet aggregation, induced by 25 mM adenosine diphosphate at concentrations, decreasing in conformity with the sequence of isolation. The IC50 of the aqueous extract was 1100 ± 30.6 mg/mL and that of BAF IV – 9.3 ± 0.45 mg/mL. When both IC50 were compared, a 118.3-fold purification of BAF IV was determined. The high biological activity of BAF IV was supposed to be due to the protein component of the fraction.
Key words: Galega officinalis L., glycoprotein fraction, medicinal plant, platelet aggregation
Barzev, G., S. Tanchev and T. Karadjov, 2003. Study on phenotypic correlations among some exterior features of growth dynamics in Hanoverian horses. Bulg. J. Vet. Med., 6, No 4, 209-214.
In 54 male Hanoverian horses, the withers height, the body length, the chest length, the chest depth, the chest width, the croup width, the croup length, the neck length, the chest girth and the cannon bone circumference were measured. All measurings were performed at the age of 30 days and 3 months and the phenotypic correlations among the exterior parameters were determined. It was observed that the body measurements related to the primary body proportions were characterized with a significant growth intensity from the age of 30 days to the age of 3 months. For the pectoral girdle, the length was characterized with relatively more intensive growth whereas for the pelvic girdle - the increase in width was relatively more pronounced. All phenotypic correlation coefficients among studied body parameters of horses were positive and their values varied from insignificant to significant. At the age of 3 years, there were significant changes in phenotypic correlation coefficients among the studied parts of the body compared to those at the age of 30 days. In the latter group, the coefficients were also positive. There was no correlation between the croup width and the other parameters.
Key words: phenotype, body correlations, exterior, body growth, horses, Hanoverian breed, conformation, performance
Haroun, E. M., T. El Mitnawi, O. H. Omer, B. H. Ali and O. M. Mahmoud, 2003. A preliminary comparative study on the efficacy of quinapyramine sulphate/chloride and melarsoprol in rats, experimentally infected with Trypanosoma evansi. Bulg. J. Vet. Med., 6, No 4, 215-221.
Using standard parasitological, pathological, haematological and sero-biochemical methods, the trypanocidal effect of quinapyramine sulphate/chloride and melarsoprol has been compared in rats experimentally infected with Trypanosoma evansi isolated from a camel. The results indicated that infection in all rats resulted in fulminating parasitaemia (> 106 trypanosomes per mL). One week after treatment, no parasitaemia was detected at necropsy in quinapyramine-treated rats but it was evident at a range of (5´105 to 5´106) in the positive control rats and in the melarsoprol-treated rats. Treatment with both drugs did not significantly influence the infection-induced changes in the PCV. The activities of ASAT and AP were significantly increased in groups II, III and IV. Treatment with melarsoprol insignificantly increased the concentration of urea in serum. There were no consistent differences in the overall histopathological pictures of the vital organs of infected and untreated rats as well as those treated with either drug following infection. It was concluded that, at the doses used, quinapyramine was effective in treating the strain of T. evansi used in this work while melarsoprol was not.
Key words: melarsoprol, quinapyramine sulphate/chloride, rats, Trypanosoma evansi
Georgieva, S. and P. Georgiev, 2003. Dose- and gender-dependence of hormonal response following external gamma irradiation in pigs. Bulg. J. Vet. Med., 6, No 4, 223-232.
The effect of gamma rays upon the reproductive potential of male and female pigs from the Danubian White breed at the age of 2 months irradiated with single doses of 1.5 and 2.5 Gy was followed out up to the age of 8 months.
The controlled hormonal levels were those of luteinizing hormone (LH), follicle stimulating hormone (FSH) and testosterone (T) in male pigs; LH, FSH and 17-β estradiol – in female pigs.
The obtained results evidenced that the changes in gonadotropic hormonal levels were dose-dependent as well as gender-dependent. While low radiation doses (up to 1.5 Gy) resulted in a transient inhibiting effect upon gonadotropic hormones LH and FSH, the high doses caused a decompensation of reproductive function via the known feedback mechanism.
After irradiation with 1.5 Gy (up to the age of 4 months), the dynamics of serum T and FSH levels in male pigs was similar to the physiological one, although more feeble and with lower values: 1.20 ± 0.04 − 3.50 ± 0.4 ng/ml for testosterone; 19.1 ± 2.2 − 12.3 ± 2.1 IU/L for FSH.
At the dose of 1.5 Gy, a compensatory elevation of studied hormonal parameters occurred at the end of the experimental period: 4.2 ± 0.5 ng/mL and 14.7 ±2.1 IU/L for T and FSH, respectively.
17-β estradiol concentrations, as an indice of follicle activity, were 58 ± 4.3 pmol/L 4 months after irradiation at 1.5 Gy, that was statistically significantly lower vs 165 ± 6 pmol/L in controls. At the age up to the 8th month, a tendency towards compensation of the harmful impact of the 1.5 Gy irradiation was observed.
Key words: irradiation, hormonal profile, pigs, reproduction
Karakolev, R., G. Monov, M. Doktorova and M. Boteva, 2003. Findings of Listeria monocytogenes in duck and goose meat and liver. Bulg. J. Vet. Med., 6, No 4, 233 -236.
Two hundred and fifty seven samples (112 samples of goose meat and liver and 145 samples duck meat and liver) were analysed. From meat and liver of fattened geese, 40 Listeria monocytogenes isolates (35.7%) - 35 from serogroup 1 and 5 - from serogroup 2 and 6 L. innocua isolates (5.36%) were obtained. From duck meat and liver, L. monocytogenes organisms were isolated in 7 samples (4.8%), all of them belonging to serogroup 1. Furthermore, there were two single samples infected with L. innocua and L. grayi.
Key words: duck, geese, Listeria monocytogenes, meat contaminations
Dinev, I., I. Borissov and D. Bakalov, 2003. Incidence of canine neoplasms - a retrospective histopathological study. IV. Oropharyngeal tumours. Bulg. J. Vet. Med., 6, No 4, 237-244.
A histopathological study of 65 specimens from spontaneous tumour growths localized in the oropharyngeal region in dogs was performed at the Faculty of Veterinary Medicine, Trakia University - Stara Zagora. The tumours were classified according to the criteria of the World Health Organization.
Twenty nine tumours (44.62%) were classified as epulides, 16 (24.62%) as malignant melanomas, 8 (12.31%) - as squamous cell carcinomas, 5 (7.69%) - as liposarcomas, 3 (4.62%) - as fibrosarcomas, 3 (4.62%) - as osteosarcomas and 1 (1.54 %) – as benign melanoma. The incidence of oral tumours depending on dog’s breed, gender and age was also studied.
Key words: dogs, incidence of oral neoplasms