Bulgarian Journal of Veterinary Medicine


ISSN 1311-1477 (print);  ISSN 131-3543 (online)

Bulgarian Journal of Veterinary Medicine
VOL. 13, No 1, MARCH 2010

 

 CONTENTS

  

 

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text

T. Mircheva Georgieva

Similarities and differences between haptoglobin
in mammals and PIT 54 in poultry – a review


Bulg. J. Vet. Med., 13, No 1, pp. 1-12                   

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R. Dimitrov, P. Yonkova, K. Stamatova, D. Yovchev & N. Tsandev

LOCALIZATION AND ACTIVITY OF TISSUE LIPOPROTEIN LIPASE IN
FELINE PROSTATE GLAND


Bulg. J. Vet. Med., 13, No 1, pp. 13-17                   

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H. MOMTAZ & S. NEJAT

DETECTION OF PROVIRAL SEQUENCES OF EQUINE INFECTIOUS ANEMIA VIRUS IN PERIPHERAL BLOOD CELLS OF HORSES IN IRAN

Bulg. J. Vet. Med.,
13, No 1, pp. 18-22                   

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E. RAHIMI, M. AMERI, H. R. KAZEMEINI & M. ELBAGI

PREVALENCE AND ANTIMICROBIAL RESISTANCE OF SALMONELLA ISOLATED FROM RETAIL RAW TURKEY, OSTRICH, AND PARTRIDGE
MEAT IN IRAN


Bulg. J. Vet. Med., 13, No 1, pp. 23-30                   

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H. DASKALOV, S. ATANASOVA, Т. STOYANCHEV & N. CHIPILEV

MICROBIOLOGICAL AND NEAR INFRARED SPECTROSCOPY ANALYSIS
OF BULGARIAN YELLOW CHEESE, CONTAMINATED WITH
LISTERIA MONOCYTOGENES


Bulg. J. Vet. Med., 13, No 1, pp. 31-37                   

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M. M. HADIPOUR

HISTOPATHOLOGICAL STUDY OF A/CHICKEN/IRAN/772/99 (H9N2)
INFLUENZA VIRUS IN COMMERCIAL BROILER CHICKENS


Bulg. J. Vet. Med., 13, No 1, pp. 38-44                   

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S. NAZIFI, M. ANSARI-LARI, M. R. TABANDEH, K. BADIEI, N. GHAFARI,
I. KARACHI, A. NOWROOZI-ASL & S. M. RAZAVI


CLINICAL RELEVANCE OF SERUM SIALIC ACIDS EVALUATION AND CORRELATION WITH HAPTOGLOBIN AND SERUM AMYLOID A IN
DISEASED CATTLE


Bulg. J. Vet. Med., 13, No 1, pp. 45-54                   

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S. P. SABEV, D. T. KANAKOV & P. I. PETKOV

ELECTROCARDIOGRAPHIC RESPONSE TO EXERCISE IN RACE HORSES DURING THE TRAINING SEASON

Bulg. J. Vet. Med., 13, No 1, pp. 55-60                   

Published by the Faculty of Veterinary Medicine, Trakia University,
6000 Stara Zagora, Bulgaria

 

 

Volume 13, Number 1, March 2010, Summaries

 

Mircheva Georgieva, T., 2010. Similarities and differences between haptoglobin in mammals and PIT 54 in poultry – a review. Bulg. J. Vet. Med., 13, No 1, 1-12.

The acute phase response (APR) is a natural systemic reaction raised to neutralize the effect of challenging agents (infections, traumas, neoplasms, immune disorders) that impair the homeostasis. Acute phase proteins (APP) are a component of APR. The purpose of the present review is to summarize the available information about haptoglobin (Нр) in mammals and its analogue  PIT 54  in Gallus gallus domesticus. Hp is a major APP in ruminants and a representative of moderate APP in men, carnivores, horses and rabbits. Chicken PIT 54 is a soluble mеmber of the so-called scavenger receptor cystein rich proteins family. Нр is an α2-glycoprotein that belongs to transport (metal-binding) conjugated proteins. The primary function of Hp is to bind free haemoglobin and thus, to protect the orga­nism from losing iron. The peroxidase activity of Hb is at the basis of assay  methods for this protein.  The review presents data about the development of knowledge about mammalian Hp and analysis of its biochemical properties. A special attention is paid on the information relation to biosynthesis and properties of mammalian Hp and the avian haemoglobin-binding protein called PIT 54. The investigation of the latter is essential for the timely detection of subclinical infections.

Key words: function, haptoglobin, PIT 54, properties

 

Dimitrov, R., P. Yonkova, K. Stamatova, D. Yovchev & N. Tsandev, 2010. Localization and activity of tissue lipoprotein lipase in feline prostate gland. Bulg. J. Vet. Med., 13, No 1, 13-17.

Tissue lipoprotein lipase (LPL) activity has been studied in prostate glands of 7 adult male European shorthair cats on cryostat cross sections by the Tween technique. The highest activity was observed at the luminal surface of the glandular epithelium, its apical parts, as well as in the lumen of glandular tubules of prostate body. A moderate intensity of LPL expression was found out in the basal parts of parenchymal epithelial cells. The enzyme was slightly expressed in the stroma of the gland, whereas no LPL activity was detected in the glandular capsule. The results provided evidence for a predilection in tissue LPL localization in epithelial components of the glands, as well as for higher enzyme activity in the luminal part of the epithelium, thus presuming a role of LPL in the lipid metabolism of glandular parenchyma, in the production and excretion of lipid products into the tubuloalveolar lumen.

Key words: cat, lipoprotein lipase, prostate gland

 

Momtaz, H. & S. Nejat, 2010.  Detection of proviral sequences of equine infectious anemia virus in peripheral blood cells of horses in Iran. Bulg. J. Vet. Med., 13, No 1, 18-22.

Equine infectious anemia virus (EIAV), a member of the genus Lentivirus of the family Retroviridae, causes a chronic infection with world-wide spread in the equids; it has been evidenced in horses, donkeys and mules. whole blood and serum samples (n=310) from horses over one year old from dif­ferent regions of Iran were examined. Samples were initially checked in the agar gel immunodiffusion (AGID) test and 9 cases (2.90%) had antibody against EIAV. Then, positive serum samples and 301 apparently healthy horses (negative in the AGID test) were examined by nested PCR to detect proviral DNA of EIA. PCR results showed that all positive sera and 2 of AGID-negative sera were positive.

Key words: agar gel immunodiffusion, equine infectious anemia, provirus, nested PCR

 

Rahimi, E., M. Ameri, H. R. Kazemeini & M. Elbagi, 2010. Prevalence and antimicrobial resistance of Salmonella isolated from retail raw turkey, ostrich and partridge meat in Iran. Bulg. J. Vet. Med., 13, No 1, 23-30.

The objective of this study was to determine the Salmonella prevalence, the serotypes involved and antimicrobial susceptibility patterns of Salmonella isolates recovered from retail raw turkey, ostrich, and partridge meat in Esfahan, Iran. A total of 249 samples of turkey, ostrich, and partridge meat were purchased from 8 randomly selected retail outlets from April 2006 to July 2007. All samples were evaluated for the presence of Salmonella, serotyped and tested for antimicrobial susceptibility. There was an overall Salmonella prevalence of 6.8%. The prevalence of Salmonella was statistically significantly higher in turkey meat (9.7%) than in ostrich meat (4.6%) (p ≤ 0.05). No Salmonella was isolated from partridge meat samples. Salmonella isolates recovered from turkey and ostrich meat samples were of 4 different serotypes including Salmonella enterica ser. Typhimurium, Salmonella enterica ser. Enteritidis, Salmonella enterica ser. Agona, and Salmonella enterica ser. Paratyphi B. The susceptibility of the 17 isolated strains to 12 antimicrobial drugs was determined using the disk diffusion method. Resistance to nalidixic acid was the most common finding (58.8%), followed by resistance to tetracycline (41.2%), streptomycin (29.4%), trimethoprim (23.5%), chloramphenicol (11.8%), and ciprofloxacin (5.9%). Salmonella isolates recovered from ostrich meat samples were susceptible to all 12 antimicrobial agents. To our knowledge, this is the first study on the prevalence of Salmonella in partridge meat and first report of the isolation of Salmonella spp. from retail raw turkey and ostrich meat in Iran.

Key words: antimicrobial resistance, ostrich, partridge, Salmonella, turkey

 

Daskalov, H., S. Atanasova, Т. Stoyanchev & N. Chipilev, 2010. Microbiological and near infrared spectroscopy analysis of Bulgarian yellow cheese, con­ta­minated with Listeria monocytogenes. Bulg. J. Vet. Med., 13, No 1, 31-37.

A simultaneous microbiological and near infrared spectroscopy (NIRS) analysis of Bulgarian yellow cheese, contaminated with Listeria monocytogenes has been performed. The results showed that the storage of samples at room temperature resulted in elimination of a part of microorganisms and their maintenance at a low concentration in one gramme product (101-103 CFU/g) during the entire experimental period. NIRS was found to be successful in distinguishing between contaminated and non-contaminated samples. A high coefficient of correlation was found out between the near-infrared spectra of samples and the presence of L. monocytogenes in Bulgarian yellow cheese. The results of the study allowed classifying properly the samples contaminated and non-contaminated with L. monocytogenes.

Key words: Listeria monocytogenes, near infrared spectroscopy, yellow cheese

 

Hadipour, M. M., 2010. Histopathological study of A/Chicken/Iran/772/99 (H9N2) influenza virus in commercial broiler chickens. Bulg. J. Vet. Med., 13,  No 1, 38-44.

Avian influenza outbreaks in 1998 due to H9N2 subtype of avian influenza virus (AIV) that occurred in poultry industry in Iran caused serious economic losses. The aim of this study was to investigate the pathogenesis, clinical signs, gross and histopathological findings of the chickens experimentally inoculated with A/Chicken/Iran/772/99(H9N2) influenza virus, isolated from the commercial broiler chickens of Iran with 60% mortality. Eighteen 3-week-old commercial broiler chickens were inoculated with 106 EID50 per bird with A/chicken/Iran/772/99(H9N2) avian influenza virus. On days 1, 2, 4, 6, 8 and 11 post-inoculation (PI) samples of the trachea, lungs, liver, pancreas, spleen, thymus, duodenum, kidneys, brain and bursa of  Fabricius were collected for histopathological study.  Clinically, depression, crouching, huddling, puffing, oedema of face and head, conjunctivitis and ruffled feathers were observed. In necropsy, turbidity of the thoracic and abdominal air sacs and mild congestion of the trachea and lung and mild accumulation of fibrinous exudate on the tracheal mucosa were seen. Tracheitis, pneumonia and tubulointerstitial nephritis were the most frequent histological lesions. The results indicated that the A/Chicken/Iran/772/99 (H9N2) avian influenza virus has pathogenicity for the trachea, lungs (pneumotropic) and kidneys (nephrotropic).

Key words: avian influenza, broiler chickens, H9N2 subtype, histopathology

 

Nazifi, S., M. Ansari-Lari, M. R. Tabandeh, K. Badiei, N. Ghafari, I. Karachi, A. Nowroozi-Asl &  S. M. Razavi, 2010. Clinical relevance of serum sialic acids evaluation and correlation with haptoglobin and serum amyloid A in diseased cattle. Bulg. J. Vet. Med., 13, No 1, 45-54.

The present study was conducted to evaluate the concentrations of sialic acids and their correlation with acute phase proteins (haptoglobin and serum amyloid A) in various inflammatory disorders in cattle. Data were obtained from six dairy farms in Fars province, southern Iran. Forty cows with various inflammatory diseases were examined: acute local traumatic reticuloperitonitis (TRP) (6 cases), theileriosis due to Theileria annulata (11 cases), acute metritis (6 cases), colisepticaemia (6 cases), ephemeral fever (5 cases) and pneumonia (6 cases). Ten clinically healthy adult cattle were selected as control group.  Serum haptoglobin (Hp), serum amyloid A (SAA), total sialic acid (TSA), lipid bound sialic acid (LBSA) and protein bound sialic acid (PBSA) were measured by validated standard methods. All  studied variables were statistically significantly higher in diseased animals compared to healthy ones (P≤0.001); but the magnitude of increase was considerably different among various dise­a­ses. TSA, PBSA and LBSA showed relatively consistent changes in all diseases, however serum amyloid A and haptoglobin were more prominently increased in TRP and metritis. Results showed significant correlations between TSA and PBSA in healthy cattle, TRP and pneumonia  groups. Significant correlations were also present between TSA and LBSA in TRP, pneumonia and metritis. No significant correlation was observed between haptoglobin and serum amyloid A as well as between either of these acute phase proteins and any other parameter.

Key words: cattle, inflammatory diseases, haptoglobin, serum amyloid A, sialic acid

 

Sabev, S. P.,  D. T. Kanakov & P. I. Petkov, 2010. Electrocardiographic response to exercise in race horses during the training season. Bulg. J. Vet. Med., 13, No 1, 55-60.

In the present study, electrocardiography was performed before and immediately after the end of exer­cise in five female Hanoverian horses during the training season. The apex/base lead I was used.  The influence of exercise on the amplitude and duration of the ECG complexes was recorded. Some abnormalities of cardiac rhythm (second-degree AV block, ventricular premature complexes) during rest were noted, too. Deviation in the length of P, S, R waves and the PQ interval were established after physical exertion. It was found out that exercise had no significant effect upon ECG wave amplitudes.

Key words: electrocardiography, horses, parameters